Cyanine3 tetrazine,Cy3 tetrazine
Cyanine3 tetrazine is a fluorescent dye with tetrazine moiety for a conjugation reaction called TCO (trans-cyclooctene) ligation. Actually, tetrazines react not only with trans-cyclooctenes, but also with (methyl)cyclopropenes and strained non-benzo-fused cycloalkynes.
Cyanine3 is a moderay hydrophobic dye, enabling labeling in non-aqueous environment as well as in aqueous media containing some organic co-solvent like acetonitrile or DMSO. For reactions in purely aqueous media, a sulfonated version of this reagent is available.
General properties
Appearance:
dark red solid
Mass spec M+ increment:
611.4
Molecular weight:
727.64
Molecular formula:
C40H46N7BF4O
Solubility:
good in DCM, DMSO, DMF
Quality control:
NMR 1H, HPLC-MS (95%)
Storage conditions:
Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
Spectral properties
Excitation maximum, nm:
555
ε, L⋅mol−1⋅cm−1:
150000
Emission maximum, nm:
570
Fluorescence quantum yield:
0.31
CF260:
0.04
CF280:
0.09
Cyanine3 tetrazine,Cy3 tetrazine
马来酰亚胺 Dye maleimides
Pyrene maleimide
BDP FL maleimide
Cyanine3 maleimide
Cyanine5 maleimide
Cyanine5.5 maleimide
Cyanine7 maleimide
Cyanine7.5 maleimide
Sulfo-Cyanine3 maleimide
Sulfo-Cyanine5 maleimide
伯胺 Primary amine
BDP FL amine
Cyanine3 amine
Cyanine5 amine
Cyanine5.5 amine
Cyanine7 amine
Cyanine7.5 amine
Sulfo-Cyanine5 amine
Sulfo-Cyanine7 amine
酰肼 Dye hydrazides
Pyrene hydrazide
BDP FL hydrazide
Cyanine3 hydrazide
Cyanine5 Boc-hydrazide
Cyanine5 hydrazide
Cyanine5.5 hydrazide
Cyanine7 hydrazide
Cyanine7.5 hydrazide